anti human albumin detection antibody  (R&D Systems)

Journal: EBioMedicine

Article Title: A diagnostic platform for rapid, simultaneous quantification of procalcitonin and C-reactive protein in human serum

doi: 10.1016/j.ebiom.2022.103867

Figure Lengend Snippet: Schematics of the PCT/CRP duplex lateral flow assay (LFA) and signal intensity quantification protocol. (a) Schematics of the PCT/CRP duplex LFA. A serum sample containing PCT and CRP antigens is added to the sample pad (Sizes of PCT and CRP antigens not to scale). The conjugate pad contains both PCT and CRP detection antibodies pre-conjugated with EuNPs. PCT and CRP antigens will bind to EuNP-conjugated PCT and CRP detection antibodies, respectively. As the sample flows through the nitrocellulose pad from left to right, the europium-conjugated CRP detection antibodies and PCT detection antibodies are captured by the first line (CRP line) and the second line (PCT line), respectively. Unbound conjugates are captured by the third line (control line). (b) Image processing protocol to quantify signal intensities of test and control lines. The three lines shown in the figure represent the CRP test line, the PCT test line, and the control line, from bottom to top. The bottom of the region of interest represents pixel position at 0, and its top represents pixel position at 1499. The width of the region of interest is 500.

Article Snippet: Mouse monoclonal anti-human CRP antibodies (Detection: Cat # M353, Lot # MA1293; Capture: Cat # M354, Lot # MA1459) were acquired from CalBioreagents (San Mateo, CA, USA).

Techniques: Lateral Flow Assay

Journal: EBioMedicine

Article Title: A diagnostic platform for rapid, simultaneous quantification of procalcitonin and C-reactive protein in human serum

doi: 10.1016/j.ebiom.2022.103867

Figure Lengend Snippet: Test strip images and quantification results of PCT antigens spiked in running buffer. (a) Images of test strips with PCT concentrations from 0·5 ng/mL to 100 ng/mL. The PCT test line intensity increases under rising concentrations. The control line intensity shows variation due to the different amounts of excess labelled antibody conjugation captured in the control zone under different PCT concentrations. Since the final PCT/CRP duplex test contains two test lines, the control line only validates the strip's function. Only the test lines intensities are analyzed for further quantification. (b) Relationship between PCT test line intensities and PCT concentrations. The linear fitting generates a good correlation, with an R 2 value of 0·96. Error bars represent standard deviations of the triplicate measurement means.

Article Snippet: Mouse monoclonal anti-human CRP antibodies (Detection: Cat # M353, Lot # MA1293; Capture: Cat # M354, Lot # MA1459) were acquired from CalBioreagents (San Mateo, CA, USA).

Techniques: Stripping Membranes, Conjugation Assay